1、一種耐高溫棉花雜交種的選育方法 

申請（專利）號：200710086690.7摘要：一種(zhong)(zhong)(zhong)(zhong)耐(nai)高(gao)溫棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)選(xuan)(xuan)育(yu)(yu)(yu)方(fang)(fang)(fang)法，屬農作物抗逆(ni)育(yu)(yu)(yu)種(zhong)(zhong)(zhong)(zhong)領(ling)域。該(gai)選(xuan)(xuan)育(yu)(yu)(yu)方(fang)(fang)(fang)法如下：1）選(xuan)(xuan)擇親本，進行(xing)(xing)雜(za)(za)交，獲(huo)得棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)F1；2）按隨機區(qu)組試驗設計種(zhong)(zhong)(zhong)(zhong)植(zhi)棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)F1，在(zai)田間調(diao)查單(dan)株結鈴數(shu)和(he)成鈴率；3）室內離體花(hua)(hua)(hua)粉培養測定(ding)棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)F1的(de)(de)(de)花(hua)(hua)(hua)粉萌發(fa)率和(he)花(hua)(hua)(hua)粉管生長(chang)長(chang)度；4）采(cai)用主成分(fen)分(fen)析方(fang)(fang)(fang)法進行(xing)(xing)篩選(xuan)(xuan)，根據步驟2）和(he)3）獲(huo)得的(de)(de)(de)結果進行(xing)(xing)主成分(fen)分(fen)析，然后(hou)以主成分(fen)分(fen)析后(hou)的(de)(de)(de)第一和(he)第二主分(fen)量數(shu)值對所測定(ding)的(de)(de)(de)棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)F1進行(xing)(xing)篩選(xuan)(xuan)。用本發(fa)明方(fang)(fang)(fang)法培育(yu)(yu)(yu)出結鈴性強(qiang)、高(gao)產和(he)適應性強(qiang)的(de)(de)(de)耐(nai)高(gao)溫棉(mian)花(hua)(hua)(hua)雜(za)(za)交種(zhong)(zhong)(zhong)(zhong)，解決例如我國長(chang)江流域雜(za)(za)交棉(mian)花(hua)(hua)(hua)種(zhong)(zhong)(zhong)(zhong)植(zhi)區(qu)域在(zai)7月至8月份期間常(chang)出現高(gao)溫逆(ni)境而導致蕾鈴脫落，并嚴重影響皮(pi)棉(mian)產量和(he)品質(zhi)的(de)(de)(de)問題。 

2、超聲波輔助農桿菌轉化棉花胚芽的方法 

申(shen)請（專利(li)）號：200710063650.0 

摘要：本發(fa)明(ming)(ming)(ming)屬于(yu)棉(mian)花轉(zhuan)(zhuan)基(ji)(ji)因(yin)(yin)技(ji)術領域，公開(kai)了超聲(sheng)波輔助農桿菌(jun)轉(zhuan)(zhuan)化棉(mian)花胚(pei)芽(ya)的(de)培育轉(zhuan)(zhuan)基(ji)(ji)因(yin)(yin)棉(mian)花的(de)新(xin)方法。包(bao)括：將(jiang)(jiang)剝掉種(zhong)皮消毒后棉(mian)種(zhong)接種(zhong)到(dao)無(wu)菌(jun)苗萌發(fa)培養(yang)基(ji)(ji)上(shang)，將(jiang)(jiang)剛萌發(fa)兩片(pian)子(zi)葉剝離，置潤濕的(de)無(wu)菌(jun)濾紙上(shang)，將(jiang)(jiang)10ml0.5OD600mm的(de)農桿菌(jun)菌(jun)液加入到(dao)塑(su)料離心管(guan)或(huo)(huo)玻璃小三角瓶(ping)中(zhong)并將(jiang)(jiang)剝好的(de)胚(pei)芽(ya)加到(dao)農桿菌(jun)液中(zhong)，將(jiang)(jiang)離心管(guan)或(huo)(huo)者小三角瓶(ping)放(fang)在(zai)(zai)超聲(sheng)波清洗器中(zhong)的(de)中(zhong)央(yang)，用(yong)100～150W的(de)超聲(sheng)波處理1-5min，將(jiang)(jiang)處理過的(de)胚(pei)芽(ya)轉(zhuan)(zhuan)移(yi)至共培養(yang)培養(yang)基(ji)(ji)培養(yang)，再用(yong)含頭孢霉素(su)的(de)無(wu)菌(jun)水沖洗，吸干胚(pei)芽(ya)表(biao)面水分，轉(zhuan)(zhuan)移(yi)至恢復培養(yang)基(ji)(ji)上(shang)，在(zai)(zai)光照下恢復培養(yang)得(de)(de)到(dao)幼芽(ya)，將(jiang)(jiang)其轉(zhuan)(zhuan)到(dao)選(xuan)擇培養(yang)基(ji)(ji)繼代，得(de)(de)到(dao)抗性(xing)芽(ya)，將(jiang)(jiang)其切下轉(zhuan)(zhuan)移(yi)至生(sheng)(sheng)(sheng)根(gen)培養(yang)基(ji)(ji)上(shang)誘導生(sheng)(sheng)(sheng)根(gen)或(huo)(huo)嫁接在(zai)(zai)棉(mian)花砧木上(shang)，得(de)(de)到(dao)再生(sheng)(sheng)(sheng)轉(zhuan)(zhuan)基(ji)(ji)因(yin)(yin)植(zhi)株(zhu)。本發(fa)明(ming)(ming)(ming)將(jiang)(jiang)GFP（綠色熒光蛋(dan)白）基(ji)(ji)因(yin)(yin)在(zai)(zai)胚(pei)芽(ya)的(de)瞬(shun)時(shi)表(biao)達率(lv)提高100多倍；首次實現了海島棉(mian)（長(chang)絨棉(mian)）的(de)轉(zhuan)(zhuan)基(ji)(ji)因(yin)(yin)植(zhi)株(zhu)；獲(huo)得(de)(de)了一批轉(zhuan)(zhuan)抗蟲基(ji)(ji)因(yin)(yin)的(de)棉(mian)花植(zhi)株(zhu)。分子(zi)及田間(jian)實驗檢測表(biao)明(ming)(ming)(ming)，外源(yuan)基(ji)(ji)因(yin)(yin)已經穩定轉(zhuan)(zhuan)入到(dao)受體(ti)棉(mian)花基(ji)(ji)因(yin)(yin)組中(zhong)并能穩定表(biao)達和遺傳。 

3、光敏核不育棉花基因資源及其應用技術 

申請（專利）號：200610124597.6 

摘要：本發(fa)明光敏核(he)不(bu)育(yu)棉(mian)花(hua)(hua)基(ji)因資(zi)源及其應用(yong)技術，在(zai)特(te)殊生態環境下：在(zai)夜間(jian)燈光照(zhao)(zhao)射不(bu)止，長期石油油泥油氣的污染農田(tian)中，將多個(ge)棉(mian)花(hua)(hua)品種(zhong)(zhong)進(jin)行種(zhong)(zhong)植，誘導出一變異單株，只結有2個(ge)晚秋桃，對其留后，經過(guo)多年持續不(bu)斷篩選，獲(huo)得光敏核(he)不(bu)育(yu)棉(mian)花(hua)(hua)基(ji)因資(zi)源，給棉(mian)花(hua)(hua)雜交制種(zhong)(zhong)開辟了新(xin)的育(yu)種(zhong)(zhong)方法和利(li)用(yong)途徑，其特(te)性為日照(zhao)(zhao)時數≥13.6nr，表(biao)現雄性不(bu)育(yu)，日照(zhao)(zhao)時數≥13.2nr表(biao)現雄性可(ke)育(yu)，不(bu)育(yu)期內可(ke)用(yong)作母(mu)本，不(bu)需去(qu)雄直接授粉(fen)雜交制種(zhong)(zhong)，可(ke)育(yu)期內可(ke)自交繁(fan)殖種(zhong)(zhong)子，適宜我國大部(bu)分(fen)棉(mian)花(hua)(hua)制種(zhong)(zhong)基(ji)地配(pei)組使(shi)用(yong)。 

4、棉花品種人工網室抗棉蚜鑒定方法 

申請（專利(li)）號：200610037612.3 

摘要(yao)：本發明(ming)涉及一種(zhong)(zhong)(zhong)(zhong)以人工(gong)(gong)網室為(wei)(wei)鑒定場所的棉(mian)(mian)(mian)(mian)花(hua)品(pin)種(zhong)(zhong)(zhong)(zhong)抗(kang)棉(mian)(mian)(mian)(mian)蚜(ya)(ya)鑒定的改良(liang)方(fang)(fang)法(fa)。該方(fang)(fang)法(fa)具體操作(zuo)步(bu)驟為(wei)(wei)：根(gen)(gen)據鑒定規模建立人工(gong)(gong)網室；冬季(ji)深翻土(tu)壤，利用低溫凍害(hai)殺死蟲卵(luan)；開(kai)春(chun)后在(zai)人工(gong)(gong)網室內播種(zhong)(zhong)(zhong)(zhong)常規棉(mian)(mian)(mian)(mian)品(pin)種(zhong)(zhong)(zhong)(zhong)，建立棉(mian)(mian)(mian)(mian)蚜(ya)(ya)繁(fan)殖(zhi)行(xing)；當棉(mian)(mian)(mian)(mian)苗(miao)普(pu)遍(bian)達到3葉(xie)期(qi)時，將供(gong)試(shi)材料(liao)分(fen)別栽入人工(gong)(gong)網室棉(mian)(mian)(mian)(mian)蚜(ya)(ya)繁(fan)殖(zhi)行(xing)之間(jian)，進(jin)行(xing)接蚜(ya)(ya)處理(li)；分(fen)別于苗(miao)蚜(ya)(ya)期(qi)、伏蚜(ya)(ya)期(qi)及秋蚜(ya)(ya)期(qi)調查供(gong)試(shi)品(pin)種(zhong)(zhong)(zhong)(zhong)的蚜(ya)(ya)害(hai)級別；以常規品(pin)種(zhong)(zhong)(zhong)(zhong)為(wei)(wei)對(dui)照，計(ji)算蚜(ya)(ya)害(hai)指數(shu)及其(qi)比對(dui)照減退率(lv)；建立棉(mian)(mian)(mian)(mian)花(hua)品(pin)種(zhong)(zhong)(zhong)(zhong)對(dui)棉(mian)(mian)(mian)(mian)蚜(ya)(ya)的抗(kang)性分(fen)級標準(zhun)，根(gen)(gen)據各供(gong)試(shi)品(pin)種(zhong)(zhong)(zhong)(zhong)的蚜(ya)(ya)害(hai)指數(shu)比對(dui)照減退率(lv)，確定其(qi)對(dui)棉(mian)(mian)(mian)(mian)蚜(ya)(ya)的抗(kang)性水平(ping)。本發明(ming)能夠(gou)顯著地提高(gao)棉(mian)(mian)(mian)(mian)花(hua)品(pin)種(zhong)(zhong)(zhong)(zhong)抗(kang)棉(mian)(mian)(mian)(mian)蚜(ya)(ya)鑒定的準(zhun)確率(lv)和可靠性。 

5、棉花的二個DREB類轉錄因子及其編碼基因與應用 

申請（專利）號：200610012084.6 

摘要：本發明公開了一(yi)(yi)個棉(mian)花的DREB類轉(zhuan)錄因子(zi)及其(qi)(qi)編碼基(ji)因與應用(yong)(yong)。其(qi)(qi)目的是提(ti)供一(yi)(yi)個來源于棉(mian)花的DREB類轉(zhuan)錄因子(zi)及其(qi)(qi)編碼基(ji)因與其(qi)(qi)在培育抗(kang)逆(ni)性(xing)(xing)提(ti)高的植物(wu)中(zhong)的應用(yong)(yong)。該DRIB類轉(zhuan)錄因子(zi)是下述氨(an)基(ji)酸殘(can)(can)基(ji)序列(lie)之一(yi)(yi)：1）序列(lie)表(biao)中(zhong)的SEQID№：1；2）將序列(lie)表(biao)中(zhong)SEQID№：1的氨(an)基(ji)酸殘(can)(can)基(ji)序列(lie)經過一(yi)(yi)至十(shi)個氨(an)基(ji)酸殘(can)(can)基(ji)的取代(dai)、缺失或添(tian)加且具有轉(zhuan)錄激活功能的調控植物(wu)抗(kang)逆(ni)性(xing)(xing)的蛋白(bai)質。本發明可應用(yong)(yong)于植物(wu)抗(kang)性(xing)(xing)的基(ji)因工程改良中(zhong)，具有較高的實(shi)際(ji)應用(yong)(yong)價值。 

6、以葵花為砧木的棉花稼接栽培方法 

申請（專利）號：200510094541.6 

摘要：以葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)為砧(zhen)木(mu)的(de)(de)棉(mian)(mian)(mian)花(hua)(hua)(hua)(hua)(hua)(hua)稼接栽(zai)(zai)培方法(fa)(fa)，其(qi)特征是在清明至立(li)夏期間，先育葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)苗(miao)(miao)；待葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)苗(miao)(miao)長出(chu)4～6片葉(xie)時(shi)，進行(xing)棉(mian)(mian)(mian)籽(zi)催芽(ya)；以葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)為砧(zhen)術，將(jiang)(jiang)葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)在地(di)表以上、底部(bu)葉(xie)片以下的(de)(de)莖(jing)部(bu)切(qie)(qie)口，切(qie)(qie)口深至木(mu)質部(bu)，將(jiang)(jiang)經過催芽(ya)的(de)(de)棉(mian)(mian)(mian)籽(zi)放入葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)切(qie)(qie)口中(zhong)，對切(qie)(qie)口部(bu)位捆綁；棉(mian)(mian)(mian)籽(zi)出(chu)苗(miao)(miao)后，將(jiang)(jiang)葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)在切(qie)(qie)口上部(bu)切(qie)(qie)頭，形成(cheng)具有葵(kui)花(hua)(hua)(hua)(hua)(hua)(hua)根莖(jing)的(de)(de)棉(mian)(mian)(mian)花(hua)(hua)(hua)(hua)(hua)(hua)苗(miao)(miao)；育苗(miao)(miao)五(wu)天以后，按常規方式將(jiang)(jiang)稼接完(wan)成(cheng)的(de)(de)棉(mian)(mian)(mian)花(hua)(hua)(hua)(hua)(hua)(hua)苗(miao)(miao)向(xiang)大田移栽(zai)(zai)，并實施田間管(guan)理，直(zhi)至采(cai)摘收獲。本發明方法(fa)(fa)可(ke)大幅度提高棉(mian)(mian)(mian)花(hua)(hua)(hua)(hua)(hua)(hua)產量(liang)、增強棉(mian)(mian)(mian)花(hua)(hua)(hua)(hua)(hua)(hua)抗病蟲害、抗自(zi)然災害能力。 

7、棉花的一種扦插繁殖方法 

申請（專利(li)）號：200510088964.7 

摘要(yao)：本(ben)發(fa)明公(gong)開了(le)一種棉(mian)(mian)花(hua)的扦(qian)插繁殖方(fang)法。該方(fang)法包括以下步驟：1）將棉(mian)(mian)花(hua)枝(zhi)條接到棉(mian)(mian)花(hua)再(zai)生(sheng)(sheng)苗(miao)(miao)(miao)(miao)(miao)生(sheng)(sheng)根(gen)(gen)培養基(ji)中進行(xing)擴(kuo)(kuo)繁生(sheng)(sheng)根(gen)(gen)，或(huo)將棉(mian)(mian)花(hua)枝(zhi)條的基(ji)部先用棉(mian)(mian)花(hua)枝(zhi)條扦(qian)插生(sheng)(sheng)根(gen)(gen)劑浸泡(pao)20～28小時，再(zai)扦(qian)插于棉(mian)(mian)花(hua)無(wu)(wu)土(tu)(tu)育(yu)(yu)苗(miao)(miao)(miao)(miao)(miao)基(ji)質中進行(xing)生(sheng)(sheng)根(gen)(gen)培養；2）待棉(mian)(mian)花(hua)枝(zhi)條長出根(gen)(gen)系后移(yi)栽，得到棉(mian)(mian)花(hua)植株。本(ben)發(fa)明具有下述優(you)點(dian)：一是(shi)可(ke)以利用棉(mian)(mian)花(hua)枝(zhi)條、贅壓進行(xing)擴(kuo)(kuo)繁；二是(shi)獲得的快(kuai)繁苗(miao)(miao)(miao)(miao)(miao)根(gen)(gen)多、苗(miao)(miao)(miao)(miao)(miao)壯、苗(miao)(miao)(miao)(miao)(miao)病少或(huo)無(wu)(wu)，取苗(miao)(miao)(miao)(miao)(miao)后根(gen)(gen)系保(bao)存完整；三(san)是(shi)可(ke)實(shi)行(xing)工廠化育(yu)(yu)苗(miao)(miao)(miao)(miao)(miao)，育(yu)(yu)苗(miao)(miao)(miao)(miao)(miao)基(ji)質質地無(wu)(wu)土(tu)(tu)，重量輕，能夠開展工廠化集中保(bao)護(hu)育(yu)(yu)苗(miao)(miao)(miao)(miao)(miao)，且風險小，效果好(hao)，節(jie)約了(le)育(yu)(yu)苗(miao)(miao)(miao)(miao)(miao)面積；四是(shi)可(ke)實(shi)行(xing)無(wu)(wu)載體(ti)單苗(miao)(miao)(miao)(miao)(miao)移(yi)栽，在無(wu)(wu)土(tu)(tu)基(ji)質床體(ti)上取苗(miao)(miao)(miao)(miao)(miao)時幼苗(miao)(miao)(miao)(miao)(miao)無(wu)(wu)載體(ti)，此外，若使用促根(gen)(gen)劑，則可(ke)加快(kuai)移(yi)栽苗(miao)(miao)(miao)(miao)(miao)生(sheng)(sheng)根(gen)(gen)速度，縮短返苗(miao)(miao)(miao)(miao)(miao)時間，提高成苗(miao)(miao)(miao)(miao)(miao)率；五是(shi)利用枝(zhi)條扦(qian)插取代棉(mian)(mian)種，解決了(le)雜(za)交(jiao)棉(mian)(mian)制種成本(ben)高、優(you)良品種易退化的難(nan)題(ti)。 

8、棉花水培漂浮育苗方法 

申請（專利(li)）號：200510031883.3 

摘要：本發明(ming)涉及一種(zhong)新型的(de)棉(mian)花育苗(miao)(miao)方(fang)法，用多孔(kong)育苗(miao)(miao)盤(pan)、基質和營養(yang)液水體苗(miao)(miao)床進行漂浮育苗(miao)(miao)，優良(liang)棉(mian)種(zhong)的(de)出苗(miao)(miao)率達到(dao)90％以上，培育出的(de)棉(mian)苗(miao)(miao)根系發達，生活(huo)力(li)強，棉(mian)苗(miao)(miao)整(zheng)齊一致，少(shao)病(bing)菌株(zhu)或無(wu)病(bing)菌株(zhu)，無(wu)雜草，成(cheng)(cheng)苗(miao)(miao)率高(gao)。與(yu)之相配套的(de)移栽(zai)技術，具(ju)有省(sheng)工，節省(sheng)成(cheng)(cheng)本，移栽(zai)簡易，取苗(miao)(miao)運苗(miao)(miao)方(fang)便，移栽(zai)成(cheng)(cheng)活(huo)率高(gao)，返苗(miao)(miao)期(qi)短，移栽(zai)時間靈活(huo)性等好特點。